Journal: Molecular Medicine
Article Title: Melatonin alleviates sepsis-induced acute lung injury by inhibiting necroptosis via reducing circulating mtDNA release
doi: 10.1186/s10020-025-01228-z
Figure Lengend Snippet: Melatonin Mitigates Septic ALI by Reducing Circulating mtDNA Release and Inflammatory Injury. ( A ) Survival rates of mice with or without melatonin therapy 24 h after CLP (log-rank [Mantel-Cox] test, n = 20). ( B ) Lung wet/dry ratio in mice with or without melatonin therapy at 24 h after CLP. ( C ) BALF protein levels in mice with or without melatonin therapy at 24 h after CLP. ( D ) Representative H&E-stained photomicrographs of lung tissues from Sham, Sham + Mel, CLP, and CLP + Mel groups. Bar = 25 μm (original magnification ×100, ×400). ( E ) Lung injury scores from the Sham, Sham + Mel, CLP, and CLP + Mel groups. ( F-J ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in Sham, Sham + Mel, CLP, and CLP + Mel groups. ( K-O ) BALF levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in the same groups. ( P-R ) qPCR analysis of mt-Col, mt-Dloop, and mMito mRNA levels in indicated tissues. Data are presented as mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test
Article Snippet: Mouse Surfactant protein D ELISA KIT , Solarbio , SEKM-0196.
Techniques: Staining, Clinical Proteomics, Enzyme-linked Immunosorbent Assay